http://ruyangmicrobio.org/introduction daily 1.0 2024-07-30 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1597294346313-VHZMH09FAWJ35OLR3BL8/110416_UC+Davis+020.JPG 简介 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1597294348135-NDFKJBMPN2RCCF5AI4NX/111021_LBL+runaround+001.JPG 简介 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1597294350311-OSVOE68CMVQ3AHF46JKH/Cindy+bady+shower_110908+014.JPG 简介 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1597294349924-WE0G16L61VSN2VRQK737/Eva1_07.jpg 简介 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1597294350313-0URQ5Z5XEQ5RMUDMJYM7/Eva1_09.jpg 简介 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1597294351912-JKD3EOWSRSHTK12FEACR/Eva1_11.jpg 简介 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1597294352187-UCEPVGELH9WAU5UMG9CU/Image0008-HF1-with+Cr-day+13.jpg 简介 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实时定量PCR/RT-PCR https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1599676789837-6H0HQIBGOSEXUSTEG2KY/qPCR+standards+curve.png 实时定量PCR/RT-PCR http://ruyangmicrobio.org/cellulose-degradation daily 0.75 2020-09-15 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383085744299-DFMNV95SZOD2M04PX7B1/Eva1_09.jpg 纤维素的微生物降解 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383085746127-JMIT2DWPPF9Z7A1TWD8W/Eva1_11.jpg 纤维素的微生物降解 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383181230105-7DMPOSKPIOPZCG2O6XFV/Eva1_01.jpg 纤维素的微生物降解 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383181231611-6OXE1T8WV3FC4RWR1MCH/Eva1_04.jpg 纤维素的微生物降解 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383181230576-T18C1HVDFRGIBOGMF7VQ/Eva1_07.jpg 纤维素的微生物降解 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383181230949-80U6Y8UJOZSADMPAFICX/Eva1_08.jpg 纤维素的微生物降解 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383181233142-TEBOE6J0INBDOMDW4X51/Eva1_12.jpg 纤维素的微生物降解 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383181233659-V390WLCSC33P6NRHGRY6/Eva1_13.jpg 纤维素的微生物降解 http://ruyangmicrobio.org/contact daily 0.75 2024-08-08 http://ruyangmicrobio.org/anaerobic-rolltube daily 0.75 2020-09-12 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383501381693-ASDOPEQBUSPZSZP2C6D3/IMG_0641.jpg 厌氧微生物滚管技术操作 先设置一个无菌厌氧试管，氮气过滤后经一长针头通入试管底部，确保试管内部的厌氧环境。这个试管用于玻璃吸管的氮气置换。 将一个装有液体培养基的厌氧试管打开，氮气过滤后经一长针头通入试管底部，确保试管内部的厌氧环境。这个试管装有新鲜的液体培养基可供细菌生长。 全程应为严格的无氧无菌操作。分别将火焰置于两个试管旁边。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383501397386-N4PV7TTVX1ZBW35X4C2R/IMG_0644.jpg 厌氧微生物滚管技术操作 厌氧菌落吸管由普通长尖头巴斯德吸管改制而成：将长尖头巴斯德吸管的尖端0.5-1厘米处置于火焰上方，轻度灼烧后玻璃软化，尖端自然随重力下垂，检查尖端和玻璃管内通道，如果尖端未变形，通道未堵塞，吸管便可用于厌氧菌落吸取转接。 吸管的另一端由小段棉花堵塞，以防止外界的杂菌进入吸管，然后吸管可灭菌干燥后备用。 吸管的棉花一段套上普通的橡胶头就可用于换气和菌落吸取了。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383501410942-8WN5H1FCL14EKUK2RTHX/IMG_0645.jpg 厌氧微生物滚管技术操作 将含有单个菌落的厌氧试管的橡胶塞打开，将换气后的吸管伸入至菌落上方，捏紧橡胶头，将吸管的弯尖头插入菌落位置，放松橡胶头，含有菌落或部分菌落的小块琼脂便被吸入到吸管尖头部分。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383501411113-G4B4OSAI8HZBIYRSWWDZ/IMG_0646.jpg 厌氧微生物滚管技术操作 将含有单个菌落的厌氧试管的橡胶塞打开，将换气后的吸管伸入至菌落上方，捏紧橡胶头，将吸管的弯尖头插入菌落位置，放松橡胶头，含有菌落或部分菌落的小块琼脂便被吸入到吸管尖头部分。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383501423432-EY5VCDI1UC01U0CZ6ZR8/IMG_0647.jpg 厌氧微生物滚管技术操作 将含有菌落的吸管转移至装有新鲜培养基的另一个厌氧管，反复抽吸液体培养基，以确保琼脂块进入到液体培养基中。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383501437145-HRFOG0QIQLJI5SLDUWQ1/IMG_0649.jpg 厌氧微生物滚管技术操作 仔细观察液体培养基，检查含有菌落的琼脂块是否悬浮在液培中。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383501435750-39W9TBSWI4BDDLAX42Z8/IMG_0650.jpg 厌氧微生物滚管技术操作 挑取菌落完成后，将橡胶塞用酒精消毒，然后迅速盖紧厌氧试管 。 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https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383463543086-S2DR01R5X6G0PQERHKQ3/Image0008.jpg 厌氧微生物滚管技术操作 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383463548666-PLJHNRCQX5X8L6UK3Q8H/SRB_cology_1.jpg 厌氧微生物滚管技术操作 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383463554573-T0VRJXOL7ZRU5IINSIEP/SRL13-5-1.jpg 厌氧微生物滚管技术操作 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383463559240-XNE5BYVRLVJ2OQ68S487/SRL13-5-2.jpg 厌氧微生物滚管技术操作 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454187908-835TXZS9A8PBHUZURYWV/IMG_0625.jpg 厌氧微生物滚管技术操作 涉及滚管实验的厌氧管应该是无菌无氧的，注射器和针头应该是灭菌后烘干的。 通过橡胶管的氮气是过滤除菌后通过厌氧试管的，由另一个注射器作出口，这个出口是用于接种注射器氮气置换除氧的。 将火焰置于氮气出口的附近，以确保接种过程中的无菌操作。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454202533-KH7K05S1MWC6LW1WY9U4/IMG_0627.jpg 厌氧微生物滚管技术操作 将接种注射器的针头伸入到作为氮气出口的注射器中抽吸换气3-5次。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454201288-UY97M8GXTVMZTRJZAE3U/IMG_0628.jpg 厌氧微生物滚管技术操作 将装有原始样品或富集培养物的厌氧试管的管口用酒精擦拭后晾干或蘸取少量酒精后用火焰灼烧灭菌。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454212681-HNMSEDSPQJC6HTL52XG6/IMG_0630.jpg 厌氧微生物滚管技术操作 将装有原始样品或富集培养物的厌氧试管的管口用酒精擦拭后晾干或蘸取少量酒精后用火焰灼烧灭菌。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454228573-29FIWO4HRJ0DVSDPXHN6/IMG_0632.jpg 厌氧微生物滚管技术操作 用置换过氮气的注射器吸取0.05-0.5mL样品，注入到第一个含有预置与50-55C水浴的液化后的琼脂培养基，混匀后，用一个新的注射器吸取0.5mL转接至另一个含5mL培养基的试管中进行10倍系列稀释。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454237469-XHIJCI1QCY75YRBYXA1B/IMG_0633.jpg 厌氧微生物滚管技术操作 预置与50-55C水浴的液化后的琼脂培养基。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454241520-3G7AWJQC7JI4S4Q2MKQZ/IMG_0634.jpg 厌氧微生物滚管技术操作 用置换过氮气的注射器吸取0.05-0.5mL样品，注入到第一个含有预置与50-55C水浴的液化后的琼脂培养基，混匀后，用一个新的注射器吸取0.5mL转接至另一个含5mL培养基的试管中进行10倍系列稀释。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454246681-3M1HI3I812BXOYTIX9CH/IMG_0635.jpg 厌氧微生物滚管技术操作 系列稀释完成后，将接种后的琼脂培养基轻轻上下倒置混匀后在冰水混合物中滚管至琼脂凝固。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1383454266180-M3S93DREODJ74ENBAZPX/IMG_0639.jpg 厌氧微生物滚管技术操作   系列稀释完成后，将接种后的琼脂培养基轻轻上下倒置混匀后在冰水混合物中滚管至琼脂凝固。 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1599884665449-983FVMC1Z645LUS39R3F/Image0008-HF1-with+Cr-day+13.jpg 厌氧微生物滚管技术操作 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1599884664675-HC967BBL50PVEONLNV3C/SRL13-110831-2.jpg 厌氧微生物滚管技术操作 http://ruyangmicrobio.org/crudeoil daily 0.75 2020-09-09 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1382853538570-3TV4RDNA6WAE981G1CQL/EVA5-D1.jpg 石油污染的微生物修复 https://images.squarespace-cdn.com/content/v1/526c0266e4b0ba0188caf1bd/1382853539125-04XVW3R7K0RWK03FU96S/EVA15-PY2d.jpg 石油污染的微生物修复 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